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non-linear curve fitting and one binding site hyperbola as model  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc non-linear curve fitting and one binding site hyperbola as model
    Non Linear Curve Fitting And One Binding Site Hyperbola As Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one-site+binding+model+(hyperbola)/nonlinear+regression++one+site+hyperbolic+binding+model/pmc09823104-317-29-39
    Average 90 stars, based on 1 article reviews
    non-linear curve fitting and one binding site hyperbola as model - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Sigma-1 Receptor Positron Emission Tomography: A New Molecular Imaging Approach Using ( S )-(-)-[ 18 F]Fluspidine in Glioblastoma.
    Article Snippet: .. The Bmax and the KD values were estimated by a nonlinear regression model (equation: one-site binding (hyperbola)) using GraphPad Prism, Version 4.1 (GraphPad Inc., La Jolla, CA, USA). .. Cryosections of brain tumour tissue from three patients (Glioblastoma multiforme IV) were obtained using a microtome (MICROM HM560, Fisher Scientific GmbH, Schwerte, Germany), mounted on microscopy slides (SuperFrost, Thermo Scientific Menzel, Fisher Scientific GmbH, Schwerte, Germany), dried for ~2 h at room temperature, and stored at −25 ◦C until the autoradiography study.

    Article Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes
    Article Snippet: .. For determining the apparent Kd value, the decrease in AtGrxS14 fluorescence at 337 nm was plotted against AtBolA1 concentration using the one binding site hyperbola model (GraphPad Prism 6 software) and the following equation: Fluorescence intensity =Bmax*[AtBolA1]/(Kd+[AtBolA1]) where Bmax represents the fluorescence intensity of maximal binding. ..

    other:

    Article Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes
    Article Snippet: For determining the apparent K d value, the decrease in AtGrxS14 fluorescence at 337 nm was plotted against AtBolA1 concentration using the one binding site hyperbola model (GraphPad Prism 6 software) and the following equation: fluorescence intensity = B max * [AtBolA1]/( K d + [AtBolA1]), where B max represents the fluorescence intensity of maximal binding.

    Article Title: A Novel Nonnucleoside Analogue That Inhibits Human Immunodeficiency Virus Type 1 Isolates Resistant to Current Nonnucleoside Reverse Transcriptase Inhibitors
    Article Snippet: Efavirenz 85.9 1.56 298.5 6.77 416.6 41.55 814.9 160.71 1,016.1 107.10 1,382.1 240.47 1,608.4 22.34 2,524 0.07 a Culture medium contained basal 2% fetal bovine serum. b EC50 values in 100% human serum were derived by fitting the increases in EC50 values associated with increasing serum concentrations to a one-site binding model (hyperbola) and extrapolation to 100% serum using GraphPad Prism 4.

    Article Title: Anti-BCMA chimeric antigen receptors with fully human heavy-chain-only antigen recognition domains.
    Article Snippet: KD values were calculated using the one-site binding (hyperbola) nonlinear regression model (GraphPad Prism 7) and the MFI of BCMA-Fc-PE-bound CAR T cells was expressed as a percentage of maximum binding sites (Bmax).

    Article Title: Anti-BCMA chimeric antigen receptors with fully human heavy-chain-only antigen recognition domains
    Article Snippet: K D values were calculated using the one-site binding (hyperbola) nonlinear regression model (GraphPad Prism 7) and the MFI of BCMA-Fc-PE-bound CAR T cells was expressed as a percentage of maximum binding sites ( B max ).

    Fluorescence:

    Article Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes
    Article Snippet: .. For determining the apparent Kd value, the decrease in AtGrxS14 fluorescence at 337 nm was plotted against AtBolA1 concentration using the one binding site hyperbola model (GraphPad Prism 6 software) and the following equation: Fluorescence intensity =Bmax*[AtBolA1]/(Kd+[AtBolA1]) where Bmax represents the fluorescence intensity of maximal binding. ..

    Concentration Assay:

    Article Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes
    Article Snippet: .. For determining the apparent Kd value, the decrease in AtGrxS14 fluorescence at 337 nm was plotted against AtBolA1 concentration using the one binding site hyperbola model (GraphPad Prism 6 software) and the following equation: Fluorescence intensity =Bmax*[AtBolA1]/(Kd+[AtBolA1]) where Bmax represents the fluorescence intensity of maximal binding. ..

    Software:

    Article Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes
    Article Snippet: .. For determining the apparent Kd value, the decrease in AtGrxS14 fluorescence at 337 nm was plotted against AtBolA1 concentration using the one binding site hyperbola model (GraphPad Prism 6 software) and the following equation: Fluorescence intensity =Bmax*[AtBolA1]/(Kd+[AtBolA1]) where Bmax represents the fluorescence intensity of maximal binding. ..



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